Suberin
in roots is deposited in the cell walls of the endodermis (Casparian
bands) and the hypodermis forming apoplastic transport barriers between
the soil and roots (Schreiber and Franke 2011). Using analytical techniques, such as gas chromatography
and mass spectrometry, characterization of the chemical composition
of endodermal and hypodermal root cell walls is carried out (Schreiber 2010). Elongases and P450
hydroxylases involved in suberin biosynthesis in Arabidopsis and rice roots are analysed
by molecular biological and biochemical techniques (Ranathunge et al. 2011). In order to relate the chemical composition of endodermal
and hypodermal root cell walls to their function as apoplastic transport
barriers, radial transport of solutes (Ranathunge et al. 2011), water (Ranathunge and Schreiber
2011) and oxygen (Kotula et al. 2009) is measured in Arabidopsis and rice roots.
Isolation
of suberized Casparian bands from Clivia miniata roots.
